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Project/Study TitleStudies on seed storage and viability seed germination and pre-treatment
Date Started
Date Completed
Place of Implementation 
College, Laguna, Taggat Industries inc. 
Funding Agency 
Taggat Industries Inc. 
Proponents 
Study Leaders
-Marcelina V. Pacho
-Angelito R. Valencia
Co-Workers
-Fernando Ilagan
-Jacinto A. Aquino
 
Objectives
1. To determine the feasible method and length of time storing seeds without lowering seed viability as determined by germination test.

2. To determine the effects of some fungicidal treatment on the viability of seeds and early growth performance of seedlings at different storage periods.

3. To determine the effects of some pretreatment methods and medium in germinating seeds
Abstracts
The test seeds of narra (Pterocarpus indicus), giant ipil-ipil (Leucaena leucocephala), Albizia falcataria, Moluccan sau and yemane (Gmelina arborea) were treated with Dowicide (DG); 2.4; 9.6; 16.8; 23.96; and 31.2 (grams) per 100 grams of seeds (each level dissolved in 2 liter water) and copper chrome boron (CCB), 1.0;2.0;3.0;4.0;and 5.0 grams per 100 grams of seeds (each level dissolved in 0.095 liter water) by steep or solution soaking) method and stored for four months to determine whether such method is feasible in protecting the seeds against deterioration without adversely affecting the seed viability as determined by germination tests. Storage in room temperature, control No. 1 (C1) and refrigerator, Control No. 2 (C2) showed the highest percent germination value although they are not significantly different from DG treatment at 16.8 g and 31.2 g concentrations.

Except for the low rate exhibited by ipil-ipil seeds treated with 5 g CCB and cold storage, all other treatments and that of room temperature (Control 1) had almost the same percentage germination.

In the same case of Moluccan sau, cold storage treatment slightly increased the percent germination but is not significantly different with that of room temperature; DG at levels, 2.4 g; 9.6 g and 23.9 g and CCB at 2.0 g and 4.0 g levels. On the other hand, yemane best treatments were that of cold storage and 2.4 g and 9.6 g of DG.

No adverse affects on the seedlings were observed when the height measurement of the narra, giant ipil-Ipil, Moluccan sau, and yemane were taken two months after germination. Seedlings that germinate from chemically treated seeds did not show any significant difference in height from that of the controls.

To determine the effects of some fungicidal treatments on the viability of seeds and early growth performance of seedlings at different storage durations, giant ipil-ipil, yemane and langosig were treated by dusting method while amugis was treated by slurry method (fungicide dissolved in 20 ml water/kg seed).

Three concentration levels (depending on recommended dosage, weight for weight basis), each of Arasan (Ar), Bordeaux mixture (Bm), and Brassicol (Br) were utilized; storage periods of 0, 2, 4 and 8 months, while refrigerator and room temperature storage served as controls.

Out of 11 treatments, only levels one of Ar and Br produced the lowest germination percentage of ipil-ipil. The mean percent germination of the same test species decreased as storage period is prolonged, indicating significant differences from each other. Its height was not adversely affected by any of the various treatments but tended to increase as storage period increase.

There was no significant effect of treatments shown in the germination percentage of yemane seeds although a decrease was observed as storage period was prolonged from 0 to 8 months. Mean heights of yemane seedlings from seeds treated with three levels of Bm, level 3 of Ar and control (ref) were the highest. The height of yemane seedlings two months after germination was noted to be highest at 0 month storage period and lowest at 8 mos. storage.


Cold storage was found to be the best treatment for langosig as percent germination is concerned. Storage period tended to lower the percent germination from 0-4 months and worst as germination became zero after eight months. There was no significant difference in height (two months after germination) among treatments be it among seeds treated by fungicide as well as those by cold storage.

The objective focused to ascertain the effects of some pretreatments and suitable sowing medium on seed germination was carried out utilizing langosig seeds.

The removal of hair-like structure from the langosig seeds and blending for 10 seconds while afloat in water slightly improved percent germination.

The same pretreatment was utilized. This was followed by sowing into medium 1:1 proportion of sawdust (treated with brown rot fungus) to ordinary garden soil. This increased percent germination of langosig test seeds although results are not statistically significant.
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